WebThe invention relates to SNP loci and a detecting method for selecting heat-resistant cows, wherein the SNP loci are basic groups of a Number 6400 locus, a Number 6600 locus and a Number 6601 locus of an HSP70A1A gene of a cow. By extracting genome DNA of the cow, heat shock protein 70A1A (HSP70A1A) gene haplotype combination of the cow is … WebThe results of the real-time PCR showed that the expression of HSP70A1A mRNA in the heart was significantly higher than that in the other tissues (P<0.05). We presume that …
Hsp70 (HSP70A1A) downregulation enhances the metastatic …
Web31 mrt. 2013 · The HSP70 protein, which is encoded by the HSP70A1A gene, is a widespread protein of the HSP family and an important intracellular molecular … WebHsp70A1A promoter activity under heat shock. Nucleotide sequence analysis identified a NFκB recognition motif on Hsp70A1A promoter. Alteration of three bases in that … theatre et diner
Novel SNPs in HSP70A1A gene and the association of ... - Springer
Web26 jul. 2024 · BackgroundMany studies have shown that diabetes is often closely related to oral squamous cell carcinoma (OSCC) occurrence and metastasis. Heat shock protein 70 (Hsp70) is a molecular chaperone related to diabetes complications. This study aims to investigate the role of Hsp70 in OSCC in expression of invadopodia-associated … WebHSP70A1A gene and P5 primers that amplified b-actin gene were separated on a 2% agarose gel and then purified with Wizard PCR Preps DNA Purification System (Pro-mega). The purified cDNA fragment was cloned into a plasmid using the Eukaryotic TA Cloning Kit (Invitrogen). The cloned plasmid contained HSP70A1A cDNA fragment Web1 jun. 2024 · HSP70A1A can be used as molecular marker in genetic selection against heat stress. Abstract Polymorphisms in the 5′ regulatory and translated regions of a heat shock protein 70 kDa protein –A1 (HSP70A1A) are observed as the responsible for cellular protection against heat stress and associated with heat tolerance in dairy cows. the government will be upon his shoulders