Limonene-6-hydroxylase
WebFourteen unigenes related to monoterpenoid biosynthesis were identified, among which (+)-pulegone reductase (PR) and (−)-menthol dehydrogenase (MD) were significantly upregulated in l-menthol chemotype and (−)-limonene 6-hydroxylase was significantly upregulated in carvone chemotype. In addition, 2,599 TFs from 66 families were identified ... WebJul 1, 2000 · The oxygenation pattern of the essential oil monoterpenes of commercial mint (Mentha) species is determined by regiospecific cytochrome P450-catalyzed …
Limonene-6-hydroxylase
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WebApr 27, 2024 · The first step requires a limonene-3-hydroxylase (L3H) activity that specifically catalyzes hydroxylation of limonene at carbon atom 3. Several protein … WebSep 14, 2024 · On the other hand, the hydroxylation of C 6 of (+)-limonene by limonene-6-hydroxylase allows the formation of (+)-trans-carveol (Scheme 2). ... chemostat in the presence of toluene as an energy and carbon source showed that this strain exclusively hydroxylates d-limonene in the C 6 position, ...
WebNov 1, 2014 · In cases of enzymes with more than one unigene, the unigene with the highest abundance was taken into consideration. The number in brackets represents the number of unigenes identified for each enzyme in the pathway. LS: Limonene synthase, L6OH: Limonene-6-hydroxylase, CD: Carveol dehydrogenase. WebJan 14, 2013 · Wuest, M.; Croteau, R.B.: Hydroxylation of specifically deuterated limonene enantiomers by cytochrome P 450 limonene-6-hydroxylase reveals the mechanism of …
WebNov 11, 2024 · Cytochrome P450 limonene-6-hydroxylase and CDH have been functionally expressed in Escherichia coli 10, 11. However, when these three genes were expressed in a single E. coli strain, an extremely low level of (−)-carvone (up to 2 μM) was obtained from whole-cell biocatalysis with (−)-limonene supplementation 12 . Web4S-limonene-6-hydroxylase labeling was associated with gland cell endoplasmic reticulum, (-)-trans-isopiperitenol dehydro-genase labeling was restricted to secretory cell mitochondria, while (+)-pulegone reductase labeling occurred only in secretory cell cytoplasm. We discuss this pathway compartmentalization in relation to possible …
WebThe limonene-6-hydroxylase from Mentha spicata (CYP71D18) was used as a query in a blastp search of an EST database generated from peltate trichomes of Origanum vulgare …
The P450 spearmint (Mentha spicata) gene, CYP71D18, was codon-optimized for E. coli, and the CPR gene ATR2 of Arabidopsis thaliana was used as the native sequence. These genes were cloned into the pCDFDuet-1 vector and validated to be consistent with designed sequences. E. coli BL21(DE3) … See more Two genes were tested for CDH, one from peppermint (Mentha × piperita) and another from Rhodococcus erythropolis. First, the peppermint CDH gene, ISPD, was … See more The P450 spearmint gene, CYP71D18, the CPR gene ATR2 of A. thaliana, and the CDH peppermint gene, ISPD, were individually confirmed to be functionally … See more CDH was shown to cause by-product formation; thus, optimizing the expression ratio between P450/CPR and CDH was attempted. This experiment was … See more From the QconCAT proteome analysis results, an additional strain was constructed to represent an improved balance of P450 and CDH expression in a … See more bungay town council contactWebMar 29, 2024 · There have been only a few reports of amino acid residues controlling regiospecificity: peppermint limonene 3-hydroxylase (GenBank Accession No. AF124817, CYP71D15) and spearmint limonene 6-hydroxylase (GenBank Accession No. AF124815, CYP71D18) show 70% identity and catalyze the regiospecific hydroxylation of limonene … half up and down hairstyles for promWebJan 14, 2014 · A cytochrome P-450 (heme-thiolate) protein from peppermint (Mentha piperita). EC 1.14.14.99 created 1992 as EC 1.14.13.47, modified 2003, transferred 2024 1.14.14.99. Monoterpene biosynthesis: specificity of the hydroxylations of (-)-limonene by enzyme preparations from peppermint (Mentha piperita), spearmint (Mentha spicata), … bungay town councillorsWebOct 31, 2000 · Alignment of deduced amino acid sequences of peppermint limonene-3-hydroxylase (PM2) and spearmint limonene-6-hydroxylase (SM12). Identical residues are indicated by boxing and reverse lettering. bungay town councilWebFunction. Involved in limonene hydroxylation to a mixture of carveol and perillyl alcohol as well as in dehydrogenation of these products to carvone and perillyl aldehyde. Aromatic … half unityWebDec 10, 2004 · The ER localization of limonene-6-hydroxylase is consistent with the ER targeting, membrane insertion sequence of this enzyme, and with its demonstrated … bungay town council referendumWebN-containing derivatives that were promising as biologically active compounds were synthesized via a series of transformations of the monoterpenoid pulegone isolated from Ziziphora clinopodioides ess bungay town council website