Phospho ampk antibody cst

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August undefined, 2024

WebPolyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr172 of human AMPKα. … WebPhospho-(Ser/Thr) AMPK Substrate (P-S/T2-102) Rabbit mAb preferentially recognizes endogenous proteins and peptides bearing the LXRXXpS/pT motif. The antibody also …

Phospho-AMPKα (Thr172) Antibody Cell Signaling …

WebThe wells are washed and rabbit anti-APP (T668) antibody is used to detect phosphorylated APP. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of APP (T668) bound. WebPolyclonal Antibody for studying AMPK-alpha1 (Thr183) phosphate/AMPK-alpha2 (Thr172) phosphate. Cited in 1031 publications. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-AMPKα (Thr172) Antibody (CST #2531) is ready to ship. tsb bank plc ceo

Phospho-Bad (Ser136) (5D8) Mouse mAb Cell Signaling …

WebSep 4, 2014 · Western blot analysis using a phospho-specific antibody revealed a significant increase in phospho-Thr184-AMPK levels in head tissue lysates from ElavGS>UAS-AMPK flies upon RU486 ... Primary antibody, rabbit anti-DILP2 (a generous gift from Dr. Seung Kim), or anti-GFP (Cell Signaling Technology; D5.1, XP) was added 1:250 and incubated … WebAMP-activated protein kinase (AMPK) is a key cellular energy sensor and regulator of metabolic homeostasis. Although it is best known for its effects on carbohydrate and lipid … WebPolyclonal Antibody for studying AP-2 mu2 (Thr156) phosphate. Cited in 9 publications. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-AP2M1 (Thr156) Antibody (CST #3843) is ready to ship. tsb bank phone number to talk

Phospho-AMPKα (Thr172) Antibody Cell Signaling Technology

Phospho-IRE1 alpha (Ser724) Polyclonal Antibody - Thermo Fisher Scientific

Web® 2015 Cell Signaling Technology, Inc. Orders ff 77-1-CELL (255) orderscellsignal.com Support 77-7-TECH (2) infocellsignal.com Web ff www.cellsignal.com page 2 of 2 9957 For western blots, incubate membrane with diluted primary antibody in either 5% w/v BSA or nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight. Webetary methodology from Cell Signaling Technology for peptide enrichment by immunoprecipitation using a specific bead-conjugated antibody in conjunction with liquid tsb bank plc henry duncan house edinburghWebThe Phospho-AMPKalpha (Thr172) Antibody from Cell Signaling Technology is a Rabbit Polyclonal antibody to PRKAA1, and PRKAA2. This antibody recognizes Human, Mouse, Rat, and Non-Human Primate antigen. The Phospho-AMPKalpha (Thr172) Antibody has been validated for the following applications: Western Blot. tsb bank phone insurance

"WebThe antibody pair provided in this kit recognizes human Phospho-EGFR (pTyr1045). Storage Stability. Rapidly measure phosphorylated protein in lysates. Screen numerous different cell lysates without performing a Western Blot analysis. Minimal hands-on time, convenient, and non-radioactive material. " - Phospho ampk antibody cst

Phospho ampk antibody cst

(2532) AMPKα Antibody - Cell Signaling Technology - CiteAb

WebSnf1 localization was observed in formaldehyde-fixed cells immunostained with anti-phospho-T172-AMPK antibody (1:50, Cell Signalling) and with TRITC-conjugated anti-rabbit antibody (1:100, Life Technologies). For the colocalization experiment, Cdc3-HA was detected with 16B12 monoclonal antibody (1:200, Babco) and with FITC-conjugated anti … WebMar 25, 2024 · As an energy sensor, adenosine monophosphate-activated protein kinase (AMPK) plays an important role in activating autophagy by inhibiting mammalian target of rapamycin (mTOR) [ 15 ]. Although AMPK is a key modulator of autophagy, it is less clear whether AMPK is involved in SQSTM1-dependent autophagy to initiate clearance of Keap1 …

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WebFeb 26, 2024 · home > human > AMPK antibody This is a Validated Antibody Database (VAD) review about human AMPK, based on 7 published articles ( read how Labome selects the articles ), using AMPK antibody in all methods. It is aimed to help Labome visitors find the most suited AMPK antibody. WebAMP-activated protein kinase (AMPK) and mechanistic target of rapamycin complex 1 (mTORC1) are meta- bolic kinases that co-ordinate nutrient supply with cell growth. AMPK negatively regulates mTORC1, and mTORC1 reciprocally phosphorylates S345/7 in both AMPK a-isoforms.

WebWestern blot analysis of Phospho-IRE1 alpha (Ser724) in HeLa cells. Samples were incubated in Phospho-IRE1 alpha (Ser724) polyclonal antibody (Product # PA1-16927) using a dilution of 2.0 µg/mL followed by an anti-rabbit HRP secondary antibody. Treated (+) or untreated (-) with 10 mM DTT for 60 min to activate the UPR. WebAll lanes : Anti-AMPK alpha 1 (phospho S487) antibody (ab131357) at 1/500 dilution Lane 1 : HUVEC cell extract untreated Lane 2 : HUVEC cell extract treated with UV Predicted band size: 64 kDa. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 (phospho S487) antibody (ab131357)

WebJul 1, 2024 · Adenosine 5′-monophosphate (AMP)–activated protein kinase (AMPK), a critical cellular energy sensor that increases life span in multiple species [reviewed in ()], is one of the factors required for the enhanced longevity caused by inhibition of mitochondrial respiration in C. elegans (5–7).AMPK is activated by an elevated AMP/adenosine 5′ … WebPhospho-AMPK alpha-1,2 (Thr183, Thr172) Recombinant Polyclonal Antibody (10HCLC) Advanced Verification This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. View all (16) AMPK alpha-1,2 antibodies Datasheet Protocols Questions & Answers Tech Support Antibody Testing Data (4) Advanced …

WebAnti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) Research with confidence – consistent and reproducible results with every …

WebThe Human Phospho-Kinase Array (Catalog # ARY003B) is a rapid, sensitive, and economical tool to simultaneously detect the relative site-specific phosphorylation of 43 kinases and 2 related total proteins without performing … tsb bank plc mortgagesWebAMPK signaling pathway; 浏览更多通路>> ... Phospho-CDK9 (Thr186) Antibody detects endogenous levels of CDK9 only when phosphorylated at Thr186. RRID: AB_2844504 引用格式: Affinity Biosciences Cat# AF4440, RRID:AB_2844504. 偶联: Unconjugated. philly home garden show february 16WebMonoclonal Antibody for studying Bad (Ser99) phosphate. Cited in 1 publication. Highly specific and rigorously validated in-house, Phospho-Bad (Ser136) (5D8) Mouse … philly home investorWebThis antibody recognizes Human, Mouse, Rat, and Non-Human Primate antigen. The Phospho-AMPKalpha (Thr172) Antibody has been validated for the following applications: … philly home investor reviewsWeb使用 Phospho-AMPKα (Thr172) (40H9) Rabbit mAb（上）或 AMPKα Antibody #2532（下），对未经处理或经寡霉素处理 (0.5 µM) 的 C2C12 细胞提取物进行蛋白质印迹分析。使用 Phospho-AMPKα (T172) (40H9) Rabbit mAb，对对照（左）或经苯乙双胍处理（右）的石蜡包埋的 NCI-H228 细胞沉淀物进行免疫组织化学分析。使用 Phospho-AMPKα (Thr172) … philly home + garden show 2023WebApr 5, 2024 · The rabbit anti-AMPK antibody (D5A2), rabbit anti-SIRT1 antibody (D1D7), rabbit anti-Phospho-FoxO1 (Ser256), and rabbit anti-Phospho-AMPK (Thr172) were purchased from Cell Signaling Technology (Danvers, MA, USA). The RIPA lysate was purchased from Kangwei Century Biotechnology (Cwbio, Beijing, China). philly homeless deaths 2020 philly home inspection