Web高效的 cDNA 扩增性能. HiScript-TS 5’/3’ RACE Kit 以 RNA 为模板高效逆转全长 cDNA,并以 cDNA 为模板快速扩增其 5’末端或 3’末端。. 试剂盒提供定向改造的具有 Template … WebThe FirstChoice™ RLM-RACE Kit contains reagents and enzymes to produce 5 RLM-RACE-ready cDNA preparations, in addition to primers and nested RACE adapter primers for 100 …
FirstChoice RLM-RACE Kit - Thermo Fisher Scientific
WebFeb 16, 2024 · 2、Oligo capping method方法(又称RLM-RACE)是由日本东京大学铃木等人开发,可以更精确的定位转录起始位点。我们知道真核生物的mRNA具有一个3’尾巴和一个5’帽子结构,该技术正是利用5’端的帽子特性对全长分子进行富集和扩增。 WebOur on-going research indicates that RLM-RACE, PPM-RACE, and qRT-PCR are very effective in the verification of sequences of miRNA targets obtained by Degradome sequencing. The protocol for RLM-RACE, PPM-RACE, and qRT-PCR is rapid, effective, cheap, and can be completed within 2–3 days. less. Related articles. Based on techniques. cleveland west side moms
FirstChoice® RLM-RACE Kit---世联博研(北京)科技有限公司
WebMost modern protocols for 5′-RACE are derived from RLM-RACE (RNA ligase-mediated amplification of cDNA ends) (Fromont-Racine, Bertrand, Pictet, & Grange, 1993; Liu & Gorovsky, 1993) and call for ligation of a synthetic oligo to the 5′-ends of all RNA in the sample, whereupon reverse transcription (RT) is carried with a primer that is specific for … WebCN101519441A CN 200810101105 CN200810101105A CN101519441A CN 101519441 A CN101519441 A CN 101519441A CN 200810101105 CN200810101105 CN 200810101105 CN 200810101105 A CN200810101105 A CN 200810101105A CN 101519441 A CN101519441 A CN 101519441A Authority CN China Prior art keywords sequence plant … WebMay 3, 2024 · 通过靶基因预测得到miR528可以靶向到OsRFI2,紧接着通过5’RLM-RACE实验鉴定到靶基因的剪切位点,并且通过RNA干扰实验进一步验证得到miR528可以靶向OsRFI2,从而控制水稻开花时间。而水稻起源于热带或亚热带地区,因此在短日照条件下开 … bmo rothschild